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Restriction Endonucleases
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Aat II
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Aat II
Recognition site:
GACGT
▼
C
C
▲
TGCAG
Activity, Fermentas:
Blue+
Green+
Orange+
Red+
Yellow+ 1x
Yellow+ 2x
50-100%
20-50%
0-20%
0-20%
100%
20-50%
Activity, NEB:
NEBuffer 1
NEBuffer 2
NEBuffer 3
NEBuffer 4
0%
50%
50%
100%
Activity, Promega:
A
B
C
D
J
MULTI-CORE≥
50√75%
10√25%
<10%
<10%
100%
<10%
Recommended buffer:
Fermentas:
Y+/Tango≥
NEB:
NEBuffer 4
Promega:
Buffer J
Incubation temperature:
37╟C
Heat Inactivation:
65╟C for 20 minutes.
Links:
Aat II, Fermentas
Aat II, NEB
Promega
"Practical Molecular Biology"
http://molbiol.edu.ru
e-mail:
pmb@molbiol.edu.ru
Russian version
