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Restriction Endonucleases
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Ale I
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Ale I
Recognition site:
CACNN
▼
NNGTG
GTGNN
▲
NNCAC
Activity, NEB:
NEBuffer 1
NEBuffer 2
NEBuffer 3
NEBuffer 4
10%
20%
10%
100%
Recommended buffer:
NEB:
NEBuffer 4
Incubation temperature:
37°C
Heat Inactivation:
65°C for 20 minutes.
Links:
Ale I, NEB
"Practical Molecular Biology"
http://molbiol.edu.ru
e-mail:
pmb@molbiol.edu.ru