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Restriction Endonucleases
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Apa I
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Apa I
Recognition site:
GGGCC
▼
C
C
▲
CCGGG
Activity, Fermentas:
Blue+
Green+
Orange+
Red+
Yellow+ 1x
Yellow+ 2x
100%
20-50%
0-20%
0-20%
20-50%
0-20%
Activity, NEB:
NEBuffer 1
NEBuffer 2
NEBuffer 3
NEBuffer 4
25%
50%
0%
100%
Activity, Promega:
A
B
C
D
MULTI-CORE≥
100%
50√75%
50√75%
<10%
75√100%
Recommended buffer:
Fermentas:
Buffer B+
NEB:
NEBuffer 4 + BSA 50 mM
Promega:
Buffer A
Incubation temperature:
37╟C
Heat Inactivation:
65╟C for 20 minutes.
Links:
Apa I, Fermentas
Apa I, NEB
Promega
"Practical Molecular Biology"
http://molbiol.edu.ru
e-mail:
pmb@molbiol.edu.ru
Russian version
