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Restriction Endonucleases
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BstX I
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BstX I
Recognition site:
CCANNNNN
▼
NTGG
GGTN
▲
NNNNNACC
Activity, Fermentas:
Blue+
Green+
Orange+
Red+
Yellow+ 1x
Yellow+ 2x
20-50%
100%
100%
50-100%
50-100%
100%
Activity, NEB:
NEBuffer 1
NEBuffer 2
NEBuffer 3
NEBuffer 4
25%
100%
100%
50%
Activity, Promega:
A
B
C
D
MULTI-CORE≥
<10%
10√25%
25√50%
100%
10√25%
Recommended buffer:
Fermentas:
Buffer O+
NEB:
NEBuffer 3
Promega:
Buffer D
Incubation temperature:
55╟C
Heat Inactivation:
65╟C for 20 minutes.
Links:
BstX I, Fermentas
BstX I, NEB
Promega
"Practical Molecular Biology"
http://molbiol.edu.ru
e-mail:
pmb@molbiol.edu.ru
Russian version
