>
Restriction Endonucleases
>
Dpn I
Home page.
Calculations.
Journals.
Protocols.
Restrictases.
Download Site
Russian version
Dpn I
Recognition site:
CH
3
|
GA
▼
TC
CT
▲
AG
|
CH
3
Activity, Fermentas:
Blue+
Green+
Orange+
Red+
Yellow+ 1x
Yellow+ 2x
100%
100%
50-100%
50-100%
100%
50-100%
Activity, NEB:
NEBuffer 1
NEBuffer 2
NEBuffer 3
NEBuffer 4
100%
100%
75%
100%
Activity, Promega:
A
B
C
D
MULTI-CORE≥
50√75%
100%
75√100%
50√75%
100%
Recommended buffer:
Fermentas:
Y+/Tango≥
NEB:
NEBuffer 4
Promega:
Buffer B
Incubation temperature:
37╟C
Heat Inactivation:
80╟C for 20 minutes.
Links:
Dpn I, Fermentas
Dpn I, NEB
Promega
"Practical Molecular Biology"
http://molbiol.edu.ru
e-mail:
pmb@molbiol.edu.ru
Russian version
