EcoR I
| |
| |
| | Recognition site:
| | | G▼AATTC
CTTAA▲G | | | | | Activity, Fermentas:
| | |
| Blue+ |
Green+ |
Orange+ |
Red+ |
Yellow+ 1x |
Yellow+ 2x |
| | 0-20% |
NR(20-50)% |
100% |
100*% |
NR(100)% |
100% |
| | | |
| | Activity, NEB:
| | |
| NEBuffer 1 |
NEBuffer 2 |
NEBuffer 3 |
NEBuffer 4 |
|
| 100% |
100% |
100% |
100% |
| | |
| | Activity, Promega:
| | |
| A |
B |
C |
D |
H |
MULTI-CORE≥ |
|
| 25√50% |
50√75% |
50√75% |
50√75% |
100% |
100%2 |
| | |
| | Recommended buffer: | | |
| Fermentas: | Buffer EcoR I+ |
| NEB: | NEBuffer EcoR I |
| Promega: | Buffer H |
| | |
| | Incubation temperature: | | |
37╟C | | |
| | Heat Inactivation: | | |
65╟C for 20 minutes. |
| |
| | Links:
| | |
EcoR I, Fermentas
EcoR I, NEB
Promega |
| |
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Russian version
