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Restriction Endonucleases
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EcoR V
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EcoR V
Recognition site:
GAT
▼
ATC
CTA
▲
TAG
Activity, NEB:
NEBuffer 1
NEBuffer 2
NEBuffer 3
NEBuffer 4
50%
75%
100%
50%
Activity, Promega:
A
B
C
D
MULTI-CORE≥
10√25%
25√50%
50√75%
100%
100%
Recommended buffer:
NEB:
NEBuffer 3 + BSA 100 mM
Promega:
Buffer D
Incubation temperature:
37╟C
Heat Inactivation:
80╟C for 20 minutes.
Links:
EcoR V, NEB
Promega
"Practical Molecular Biology"
http://molbiol.edu.ru
e-mail:
pmb@molbiol.edu.ru
Russian version
