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Restriction Endonucleases
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Hind III
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Hind III
Recognition site:
A
▼
AGCTT
TTCGA
▲
A
Activity, Fermentas:
Blue+
Green+
Orange+
Red+
Yellow+ 1x
Yellow+ 2x
0-20%
20-50%
0-20%
100%
50-100%
50-100%
Activity, NEB:
NEBuffer 1
NEBuffer 2
NEBuffer 3
NEBuffer 4
50%
100%
10%
50%
Activity, Promega:
A
B
C
D
E
MULTI-CORE≥
25√50%
100%
75√100%
10√25%
100%
50√75%
Recommended buffer:
Fermentas:
Buffer R+
NEB:
NEBuffer 2
Promega:
Buffer E
Incubation temperature:
37╟C
Heat Inactivation:
65╟C for 20 minutes.
Links:
Hind III, Fermentas
Hind III, NEB
Promega
"Practical Molecular Biology"
http://molbiol.edu.ru
e-mail:
pmb@molbiol.edu.ru
Russian version
