>
Restriction Endonucleases
>
Hpa II
Home page.
Calculations.
Journals.
Protocols.
Restrictases.
Download Site
Russian version
Hpa II
Recognition site:
C
▼
CGG
GGC
▲
C
Activity, Fermentas:
Blue+
Green+
Orange+
Red+
Yellow+ 1x
Yellow+ 2x
50-100%
50-100%
0-20%
20-50%
100%
20-50%
Activity, NEB:
NEBuffer 1
NEBuffer 2
NEBuffer 3
NEBuffer 4
100%
50%
10%
100%
Activity, Promega:
A
B
C
D
MULTI-CORE≥
100%
50√75%
50√75%
10√25%
100%
Recommended buffer:
Fermentas:
Y+/Tango≥
NEB:
NEBuffer 1
Promega:
Buffer A
Incubation temperature:
37╟C
Heat Inactivation:
65╟C for 20 minutes.
Links:
Hpa II, Fermentas
Hpa II, NEB
Promega
"Practical Molecular Biology"
http://molbiol.edu.ru
e-mail:
pmb@molbiol.edu.ru
Russian version
