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Restriction Endonucleases
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Hph I
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Hph I
Recognition site:
GGTGA(N)
8
▼
CCACT(N)
7
▲
Activity, Fermentas:
Blue+
Green+
Orange+
Red+
Yellow+ 1x
Yellow+ 2x
100%
0-20%
0-20%
0-20%
20-50%
0-20%
Activity, NEB:
NEBuffer 1
NEBuffer 2
NEBuffer 3
NEBuffer 4
NR
75%
0%
100%
Recommended buffer:
Fermentas:
Buffer B+
NEB:
NEBuffer 4
Incubation temperature:
37°C
Heat Inactivation:
65°C for 20 minutes.
Links:
Hph I, Fermentas
Hph I, NEB
"Practical Molecular Biology"
http://molbiol.edu.ru
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