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Restriction Endonucleases
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Kpn I
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Kpn I
Recognition site:
GGTAC
▼
C
C
▲
CATGG
Activity, Fermentas:
Blue+
Green+
Orange+
Red+
Yellow+ 1x
Yellow+ 2x
20-50%
0-20%
0-20%
0-20%
20-50%
0-20%
Activity, NEB:
NEBuffer 1
NEBuffer 2
NEBuffer 3
NEBuffer 4
100%
75%
0%
50%
Activity, Promega:
A
B
C
D
J
MULTI-CORE≥
100%
2
25√50%
25√50%
<10%
100%
75√100%
Recommended buffer:
Fermentas:
Buffer Kpn I+
NEB:
NEBuffer 1 + BSA 10 mM
Promega:
Buffer J
Incubation temperature:
37╟C
Heat Inactivation:
No
Links:
Kpn I, Fermentas
Kpn I, NEB
Promega
"Practical Molecular Biology"
http://molbiol.edu.ru
e-mail:
pmb@molbiol.edu.ru
Russian version
