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Restriction Endonucleases
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Mnl I
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Mnl I
Recognition site:
CCTC(N)
7
▼
GGAG(N)
6
▲
Activity, Fermentas:
Blue+
Green+
Orange+
Red+
Yellow+ 1x
Yellow+ 2x
50-100%
100%
20-50%
20-50%
20-50%
20-50%
Activity, NEB:
NEBuffer 1
NEBuffer 2
NEBuffer 3
NEBuffer 4
75%
100%
50%
75%
Recommended buffer:
Fermentas:
Buffer G+
NEB:
NEBuffer 2 + BSA 50 mM
Incubation temperature:
37°C
Heat Inactivation:
65°C for 20 minutes.
Links:
Mnl I, Fermentas
Mnl I, NEB
"Practical Molecular Biology"
http://molbiol.edu.ru
e-mail:
pmb@molbiol.edu.ru
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