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Restriction Endonucleases
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MspA1 I
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MspA1 I
Recognition site:
CMG
▼
CKG
GKC
▲
GMC
Activity, NEB:
NEBuffer 1
NEBuffer 2
NEBuffer 3
NEBuffer 4
50%
100%
50%
100%
Activity, Promega:
A
B
C
D
MULTI-CORE≥
25√50%
100%
2
100%
10√25%
100%
Recommended buffer:
NEB:
NEBuffer 4 + BSA 50 mM
Promega:
Buffer C
Incubation temperature:
37╟C
Heat Inactivation:
65╟C for 20 minutes.
Links:
MspA1 I, NEB
Promega
"Practical Molecular Biology"
http://molbiol.edu.ru
e-mail:
pmb@molbiol.edu.ru
Russian version
