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Restriction Endonucleases
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Nae I
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Nae I
Recognition site:
GCC
▼
GGC
CGG
▲
CCG
Activity, NEB:
NEBuffer 1
NEBuffer 2
NEBuffer 3
NEBuffer 4
100%
75%
10%
75%
Activity, Promega:
A
B
C
D
MULTI-CORE≥
100%
50√75%
25√50%
<10%
50√75%
Recommended buffer:
NEB:
NEBuffer 1
Promega:
Buffer A
Incubation temperature:
37╟C
Heat Inactivation:
65╟C for 20 minutes.
Links:
Nae I, NEB
Promega
"Practical Molecular Biology"
http://molbiol.edu.ru
e-mail:
pmb@molbiol.edu.ru
Russian version
