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Restriction Endonucleases
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Nco I
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Nco I
Recognition site:
C
▼
CATGG
GGTAC
▲
C
Activity, Fermentas:
Blue+
Green+
Orange+
Red+
Yellow+ 1x
Yellow+ 2x
20-50%
20-50%
20-50%
50-100%
100%
100%
Activity, NEB:
NEBuffer 1
NEBuffer 2
NEBuffer 3
NEBuffer 4
100%
100%
100%
100%
Activity, Promega:
A
B
C
D
MULTI-CORE≥
50√75%
75√100%
75√100%
100%
75√100%
Recommended buffer:
Fermentas:
Y+/Tango≥
NEB:
NEBuffer 4
Promega:
Buffer D
Incubation temperature:
37╟C
Heat Inactivation:
65╟C for 20 minutes.
Links:
Nco I, Fermentas
Nco I, NEB
Promega
"Practical Molecular Biology"
http://molbiol.edu.ru
e-mail:
pmb@molbiol.edu.ru
Russian version
