>
Restriction Endonucleases
>
Nhe I
Home page.
Calculations.
Journals.
Protocols.
Restrictases.
Download Site
Russian version
Nhe I
Recognition site:
G
▼
CTAGC
CGATC
▲
G
Activity, Fermentas:
Blue+
Green+
Orange+
Red+
Yellow+ 1x
Yellow+ 2x
100%
20-50%
0-20%
0-20%
100%
0-20%
Activity, NEB:
NEBuffer 1
NEBuffer 2
NEBuffer 3
NEBuffer 4
100%
100%
10%
100%
Activity, Promega:
A
B
C
D
MULTI-CORE≥
75√100%
100%
75√100%
10√25%
100%
Recommended buffer:
Fermentas:
Y+/Tango≥
NEB:
NEBuffer 2 + BSA 50 mM
Promega:
Buffer B
Incubation temperature:
37╟C
Heat Inactivation:
65╟C for 20 minutes.
Links:
Nhe I, Fermentas
Nhe I, NEB
Promega
"Practical Molecular Biology"
http://molbiol.edu.ru
e-mail:
pmb@molbiol.edu.ru
Russian version
