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Restriction Endonucleases
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Nsi I
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Nsi I
Recognition site:
ATGCA
▼
T
T
▲
ACGTA
Activity, NEB:
NEBuffer 1
NEBuffer 2
NEBuffer 3
NEBuffer 4
25%
25%
50%
10%
Activity, Promega:
A
B
C
D
MULTI-CORE≥
10√25%
50√75%
50√75%
100%
10√25%
Recommended buffer:
NEB:
NEBuffer Nsi I
Promega:
Buffer D
Incubation temperature:
37╟C
Heat Inactivation:
65╟C for 20 minutes.
Links:
Nsi I, NEB
Promega
"Practical Molecular Biology"
http://molbiol.edu.ru
e-mail:
pmb@molbiol.edu.ru
Russian version
