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Restriction Endonucleases
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Pst I
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Pst I
Recognition site:
CTGCA
▼
G
G
▲
ACGTC
Activity, Fermentas:
Blue+
Green+
Orange+
Red+
Yellow+ 1x
Yellow+ 2x
50-100%
50-100%
100%
100%
50-100%
50-100%
Activity, NEB:
NEBuffer 1
NEBuffer 2
NEBuffer 3
NEBuffer 4
75%
75%
100%
50%
Activity, Promega:
A
B
C
D
H
MULTI-CORE≥
10√25%
50√75%
50√75%
50√75%
100%
25√50%
Recommended buffer:
Fermentas:
Buffer O+
NEB:
NEBuffer 3
Promega:
Buffer H
Incubation temperature:
37╟C
Heat Inactivation:
80╟C for 20 minutes.
Links:
Pst I, Fermentas
Pst I, NEB
Promega
"Practical Molecular Biology"
http://molbiol.edu.ru
e-mail:
pmb@molbiol.edu.ru
Russian version
