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Restriction Endonucleases
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Pvu I
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Pvu I
Recognition site:
CGAT
▼
CG
GC
▲
TAGC
Activity, Fermentas:
Blue+
Green+
Orange+
Red+
Yellow+ 1x
Yellow+ 2x
0-20%
20-50%
50-100%
100%
50-100%
100%
Activity, NEB:
NEBuffer 1
NEBuffer 2
NEBuffer 3
NEBuffer 4
10%
75%
100%
10%
Activity, Promega:
A
B
C
D
MULTI-CORE≥
10√25%
25√50%
50√75%
100%
<10%
Recommended buffer:
Fermentas:
Buffer R+
NEB:
NEBuffer 3 + BSA 100 mM
Promega:
Buffer D
Incubation temperature:
37╟C
Heat Inactivation:
80╟C for 20 minutes.
Links:
Pvu I, Fermentas
Pvu I, NEB
Promega
"Practical Molecular Biology"
http://molbiol.edu.ru
e-mail:
pmb@molbiol.edu.ru
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