Restrictase Sca I | Practical Molecular Biology

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Sca I

Recognition site:

AGT^▼ACT
TCA_▲TGA

Activity, Fermentas:

Blue+	Green+	Orange+	Red+	Yellow+ 1x	Yellow+ 2x

0-20%	0-20%	0-20%	0-20%	0-20%	0-20%

Activity, NEB:

NEBuffer 1	NEBuffer 2	NEBuffer 3	NEBuffer 4

25%	75%	50%	25%

Activity, Promega:

A	B	C	D	K	MULTI-CORE≥

<10%	100%	50√75%	75√100%	100%	10√25%²

Recommended buffer:

Fermentas:	Buffer Sca I+
NEB:	NEBuffer Sca
Promega:	Buffer K

Incubation temperature:

37╟C

Heat Inactivation:

80╟C for 20 minutes.

Links:

Sca I, Fermentas
Sca I, NEB
Promega

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