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Restriction Endonucleases
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Sfi I
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Sfi I
Recognition site:
GGCCNNNN
▼
NGGCC
CCGGN
▲
NNNNCCGG
Activity, NEB:
NEBuffer 1
NEBuffer 2
NEBuffer 3
NEBuffer 4
0%
100%
10%
100%
Activity, Promega:
A
B
C
D
MULTI-CORE≥
75√100%
100%
75√100%
25√50%
75√100%
Recommended buffer:
NEB:
NEBuffer 2 + BSA 50 mM
Promega:
Buffer B
Incubation temperature:
50╟C
Heat Inactivation:
No
Links:
Sfi I, NEB
Promega
"Practical Molecular Biology"
http://molbiol.edu.ru
e-mail:
pmb@molbiol.edu.ru
Russian version
