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Restriction Endonucleases
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Sph I
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Sph I
Recognition site:
GCATG
▼
C
C
▲
GTACG
Activity, NEB:
NEBuffer 1
NEBuffer 2
NEBuffer 3
NEBuffer 4
100%
100%
50%
100%
Activity, Promega:
A
B
C
D
K
MULTI-CORE≥
75√100%
75√100%
100%
75√100%
100%
10√25%
Recommended buffer:
NEB:
NEBuffer 2
Promega:
Buffer K
Incubation temperature:
37╟C
Heat Inactivation:
65╟C for 20 minutes.
Links:
Sph I, NEB
Promega
"Practical Molecular Biology"
http://molbiol.edu.ru
e-mail:
pmb@molbiol.edu.ru
Russian version
