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Restriction Endonucleases
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Ssp I
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Ssp I
Recognition site:
AAT
▼
ATT
TTA
▲
TAA
Activity, Fermentas:
Blue+
Green+
Orange+
Red+
Yellow+ 1x
Yellow+ 2x
20-50%
100%
0-20%
50-100%
100%
20-50%
Activity, NEB:
NEBuffer 1
NEBuffer 2
NEBuffer 3
NEBuffer 4
50%
100%
50%
50%
Activity, Promega:
A
B
C
D
E
MULTI-CORE≥
10√25%
50√75%
50√75%
75√100%
100%
50√75%
Recommended buffer:
Fermentas:
Buffer G+
NEB:
NEBuffer Ssp
Promega:
Buffer E
Incubation temperature:
37╟C
Heat Inactivation:
65╟C for 20 minutes.
Links:
Ssp I, Fermentas
Ssp I, NEB
Promega
"Practical Molecular Biology"
http://molbiol.edu.ru
e-mail:
pmb@molbiol.edu.ru
Russian version
