> Restriction Endonucleases > BstX I

BstX I


 
 
 Recognition site:
 
 CCANNNNNNTGG
GGTNNNNNNACC
 
 Activity, Fermentas:
 
Blue+ Green+ Orange+ Red+ Yellow+ 1x Yellow+ 2x
20-50% 100% 100% 50-100% 50-100% 100%
 
 
 Activity, NEB:
 
NEBuffer 1 NEBuffer 2 NEBuffer 3 NEBuffer 4
25% 100% 100% 50%
 
 Activity, Promega:
 
 
A B C D MULTI-CORE≥
<10% 10√25% 25√50% 100% 10√25%
 
 Recommended buffer: 
Fermentas:Buffer O+
NEB:NEBuffer 3
Promega:Buffer D
 
 Incubation temperature:  55╟C
 
 Heat Inactivation:  65╟C for 20 minutes.
 
 Links:
 
  BstX I, Fermentas
BstX I, NEB
Promega
 
 



"Practical Molecular Biology" http://molbiol.edu.ru
e-mail: pmb@molbiol.edu.ru


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