> Restriction Endonucleases > EcoO109 I

EcoO109 I


 
 
 Recognition site:
 
 RGGNCCY
YCCNGGR
 
 Activity, Fermentas:
 
Blue+ Green+ Orange+ Red+ Yellow+ 1x Yellow+ 2x
50-100% 20-50% 20-50% 20-50% 100% 100%
 
 
 Activity, NEB:
 
NEBuffer 1 NEBuffer 2 NEBuffer 3 NEBuffer 4
100% 100% 75% 100%
 
 Recommended buffer: 
Fermentas:Y+/Tango™
NEB:NEBuffer 4 + BSA 50mM
 
 Incubation temperature:  37°C
 
 Heat Inactivation:  65°C for 20 minutes.
 
 Links:
 
  EcoO109 I, Fermentas
 
 
 



"Practical Molecular Biology" http://molbiol.edu.ru
e-mail: pmb@molbiol.edu.ru


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