> Restriction Endonucleases > Sac I

Sac I


 
 
 Recognition site:
 
 GAGCTC
CTCGAG
 
 Activity, Fermentas:
 
Blue+ Green+ Orange+ Red+ Yellow+ 1x Yellow+ 2x
50-100% 20-50% 0-20% 0-20% 50-100% 20-50%
 
 
 Activity, NEB:
 
NEBuffer 1 NEBuffer 2 NEBuffer 3 NEBuffer 4
100% 50% 10% 100%
 
 Activity, Promega:
 
 
A B C D J MULTI-CORE≥
75√100% 25√50% 25√50% <10% 100% 100%
 
 Recommended buffer: 
Fermentas:Buffer Sac I+
NEB:NEBuffer 1 + BSA 10 mM
Promega:Buffer J
 
 Incubation temperature:  37╟C
 
 Heat Inactivation:  65╟C for 20 minutes.
 
 Links:
 
  Sac I, Fermentas
Sac I, NEB
Promega
 
 



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