> Restriction Endonucleases > Sfi I

Sfi I


 
 
 Recognition site:
 
 GGCCNNNNNGGCC
CCGGNNNNNCCGG
 
 
 Activity, NEB:
 
NEBuffer 1 NEBuffer 2 NEBuffer 3 NEBuffer 4
0% 100% 10% 100%
 
 Activity, Promega:
 
 
A B C D MULTI-CORE≥
75√100% 100% 75√100% 25√50% 75√100%
 
 Recommended buffer: 
NEB:NEBuffer 2 + BSA 50 mM
Promega:Buffer B
 
 Incubation temperature:  50╟C
 
 Heat Inactivation:  No
 
 Links:
 
 
Sfi I, NEB
Promega
 
 



"Practical Molecular Biology" http://molbiol.edu.ru
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