>
Restriction Endonucleases
>
Sma I
Home page.
Calculations.
Journals.
Protocols.
Restrictases.
Download Site
Russian version
Sma I
Recognition site:
CCC
▼
GGG
GGG
▲
CCC
Activity, Fermentas:
Blue+
Green+
Orange+
Red+
Yellow+ 1x
Yellow+ 2x
50-100%
0-20%
0-20%
0-20%
100%
0-20%
Activity, NEB:
NEBuffer 1
NEBuffer 2
NEBuffer 3
NEBuffer 4
0%
0%
0%
100%
Activity, Promega:
A
B
C
D
J
MULTI-CORE≥
<10%
<10%
<10%
<10%
100%
100%
Recommended buffer:
Fermentas:
Y+/Tango≥
NEB:
NEBuffer 4
Promega:
Buffer J
Incubation temperature:
30╟C
Heat Inactivation:
65╟C for 20 minutes.
Links:
Sma I, Fermentas
Sma I, NEB
Promega
"Practical Molecular Biology"
http://molbiol.edu.ru
e-mail:
pmb@molbiol.edu.ru
Russian version
