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Restriction Endonucleases
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Spe I
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Spe I
Recognition site:
A
▼
CTAGT
TGATC
▲
A
Activity, NEB:
NEBuffer 1
NEBuffer 2
NEBuffer 3
NEBuffer 4
75%
100%
25%
75%
Activity, Promega:
A
B
C
D
MULTI-CORE≥
75√100%
100%
75√100%
75√100%
100%
Recommended buffer:
NEB:
NEBuffer 2 + BSA 50 mM
Promega:
Buffer B
Incubation temperature:
37╟C
Heat Inactivation:
65╟C for 20 minutes.
Links:
Spe I, NEB
Promega
"Practical Molecular Biology"
http://molbiol.edu.ru
e-mail:
pmb@molbiol.edu.ru
Russian version
