> Restriction Endonucleases > Ssp I

Ssp I


 
 
 Recognition site:
 
 AATATT
TTATAA
 
 Activity, Fermentas:
 
Blue+ Green+ Orange+ Red+ Yellow+ 1x Yellow+ 2x
20-50% 100% 0-20% 50-100% 100% 20-50%
 
 
 Activity, NEB:
 
NEBuffer 1 NEBuffer 2 NEBuffer 3 NEBuffer 4
50% 100% 50% 50%
 
 Activity, Promega:
 
 
A B C D E MULTI-CORE≥
10√25% 50√75% 50√75% 75√100% 100% 50√75%
 
 Recommended buffer: 
Fermentas:Buffer G+
NEB:NEBuffer Ssp
Promega:Buffer E
 
 Incubation temperature:  37╟C
 
 Heat Inactivation:  65╟C for 20 minutes.
 
 Links:
 
  Ssp I, Fermentas
Ssp I, NEB
Promega
 
 



"Practical Molecular Biology" http://molbiol.edu.ru
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